Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system

doi: 10.1007/s10096-019-03703-y

Figure Lengend Snippet: Blood culture samples included in the study. Positive blood culture bottles were analyzed with the Accelerate PhenoTest™ BC using software version SW1.0 ( a ) or SW1.2 ( b ). ID, identification; AST, antimicrobial susceptibility testing

Article Snippet: The Accelerate PhenoTest™ BC kit (Accelerate Diagnostics, Inc., Tucson, AZ, USA) was run according to manufacturer’s instructions.

Techniques: Software

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system

doi: 10.1007/s10096-019-03703-y

Figure Lengend Snippet: Sample characteristics and technical performance of the Accelerate PhenoTest™ BC kit

Article Snippet: The Accelerate PhenoTest™ BC kit (Accelerate Diagnostics, Inc., Tucson, AZ, USA) was run according to manufacturer’s instructions.

Techniques:

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system

doi: 10.1007/s10096-019-03703-y

Figure Lengend Snippet: Identification results from Accelerate PhenoTest™ BC kit in comparison to standard methods

Article Snippet: The Accelerate PhenoTest™ BC kit (Accelerate Diagnostics, Inc., Tucson, AZ, USA) was run according to manufacturer’s instructions.

Techniques: Staining

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system

doi: 10.1007/s10096-019-03703-y

Figure Lengend Snippet: Performance in species identification Accelerate PhenoTest™ BC kit in comparison to standard methods

Article Snippet: The Accelerate PhenoTest™ BC kit (Accelerate Diagnostics, Inc., Tucson, AZ, USA) was run according to manufacturer’s instructions.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

doi: 10.1128/JCM.01329-17

Figure Lengend Snippet: Flowchart of sample disposition after reevaluation of data with the 2017 software update. Footnotes a to g in the flowchart include additional information about the categories. For footnote a, the 560 excluded samples include the following reasons for exclusion: deviation from the protocol ( n = 216), experiments halted ( n = 26), experiments never run ( n = 15), bottle received >8 h after positive result ( n = 31), Gram staining shows no organism ( n = 24), isolate not received at the reference laboratory ( n = 18), isolate received more than 4 days after medium preparation ( n = 3), ID reference growth failure ( n = 29), nonpure isolate ( n = 169), ID reference purity plate failure ( n = 6), invalid ID reference result ( n = 1), and Accelerate Pheno system run state not “complete” ( n = 22). For footnote b with monomicrobic, a single on-panel organism was reported. For footnote c with polymicrobic, this category includes polymicrobial samples where the Accelerate PhenoTest BC kit ID results exactly match the reference results. For footnote d with false-positive, the false-positive category includes monomicrobial or polymicrobial runs containing any false-positive result(s). For footnote e with indeterminate, all indeterminate samples had only indeterminate/negative results. For footnote f with unresolved for fresh samples, of the 48 fresh unresolved false-positive results, 43 showed genus-level agreement, while the remaining five were one S. aureus called CoNS, one S. aureus plus Pantoea species mix called Klebsiella spp., one Pseudomonas putida called Citrobacter spp., one Lactococcus raffinolactis called Streptococcus spp., and one Streptococcus dysgalactiae subsp. equisimilis called E. faecium by the Accelerate Pheno system. For footnote g with unresolved for seeded samples, of the 24 seeded samples containing unresolved false-positive results, 15 showed genus-level agreement, one sample gave two false-positive results, while the remaining nine were one Pantoea sample called Enterobacter spp., one C. koseri sample called Citrobacter spp. plus Proteus spp., two C. koseri samples called Citrobacter spp. plus Klebsiella spp., one C. freundii sample called Citrobacter spp. plus Enterobacter spp., one C. koseri sample called Citrobacter spp. plus E. faecium plus Klebsiella spp., one E. cloacae complex sample called Enterobacter spp. plus P. aeruginosa , one E. faecalis sample called E. faecalis plus E. faecium , and one Streptococcus pyogenes sample called Streptococcus spp. plus E. faecium by the Accelerate Pheno system.

Article Snippet: Thirteen geographically diverse U.S. clinical sites (Lewisville, TX; Iowa City, IA; Los Angeles, CA [2 sites]; Liverpool, NY; Rochester, MN; Milwaukee, WI; Columbus, OH; Gilbert, AZ; Maywood, IL; Danville, PA; Baltimore, MD; Tucson, AZ) enrolled and tested positive blood cultures (BCs) with the Accelerate Pheno system using the Accelerate PhenoTest BC kit.

Techniques: Software, Staining

Journal: Journal of Clinical Microbiology

Article Title: Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

doi: 10.1128/JCM.01329-17

Figure Lengend Snippet: Monomicrobial call performance comparison with FDA-cleared Accelerate Pheno system software and postclearance 2017 software update

Article Snippet: Thirteen geographically diverse U.S. clinical sites (Lewisville, TX; Iowa City, IA; Los Angeles, CA [2 sites]; Liverpool, NY; Rochester, MN; Milwaukee, WI; Columbus, OH; Gilbert, AZ; Maywood, IL; Danville, PA; Baltimore, MD; Tucson, AZ) enrolled and tested positive blood cultures (BCs) with the Accelerate Pheno system using the Accelerate PhenoTest BC kit.

Techniques: Comparison, Software, Staining

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Arginine vasopressin induces ferroptosis to promote heart failure via activation of the V1aR/CaN/NFATC3 pathway

doi: 10.3724/abbs.2023289

Figure Lengend Snippet: Detection of the AVP/CaN/NFATC3 pathway and ferroptosis in mice A mouse model of HF was established, and myocardial tissues were obtained from the mice for in vivo experiments. (A‒C) Western blot analysis was performed to measure the protein levels of V1aR, CaN, NFATC3, GPX4 and ACSL4. A colorimetric kit was used to measure the iron level (D) and MDA level (E). Data are expressed as the mean±SEM (n=3). **P<0.01, ***P<0.001 vs the Sham group.

Article Snippet: Colorimetric kits were employed to assess the total iron level (E-BC-K773-M; Elabscience, Wuhan, China), Fe 2+ concentration (E-BC-K881-M; Elabscience) and MDA concentration (E-BC-K025-S; Elabscience) according to the manufacturer’s instructions.

Techniques: In Vivo, Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Arginine vasopressin induces ferroptosis to promote heart failure via activation of the V1aR/CaN/NFATC3 pathway

doi: 10.3724/abbs.2023289

Figure Lengend Snippet: Detection of the AVP/CaN/NFATC3 pathway and ferroptosis in HCM cells HCM cells were used for the in vitro experiments. (A) CCK-8 assay was performed to assess the effect of different concentrations of dDAVP (10‒7, 10 ‒8, 10‒9, and 10‒10 M) on cell proliferation. (B–D) Western blot analysis was performed to examine the protein levels of V1aR, CaN, NFATC3, GPX4 and ACSL4. A colorimetric kit was used to measure the Fe2+ concentration (E) and MDA level (F). Data are expressed as the mean±SEM ( n=3). **P<0.01, ***P<0.001 vs the Ctrl group.

Article Snippet: Colorimetric kits were employed to assess the total iron level (E-BC-K773-M; Elabscience, Wuhan, China), Fe 2+ concentration (E-BC-K881-M; Elabscience) and MDA concentration (E-BC-K025-S; Elabscience) according to the manufacturer’s instructions.

Techniques: In Vitro, CCK-8 Assay, Western Blot, Concentration Assay

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Arginine vasopressin induces ferroptosis to promote heart failure via activation of the V1aR/CaN/NFATC3 pathway

doi: 10.3724/abbs.2023289

Figure Lengend Snippet: Effects of the AVP/CaN/NFATC3 pathway on ferroptosis in HCM cells HCM cells were used for the in vitro experiments. SR49059 (a V1aR inhibitor, 3×10 –6 M), CsA (a CaN inhibitor, 1 μM) and ferrostatin-1 (a ferroptosis inhibitor, 1 mM) were used to inhibit the AVP-CaN-NFATC3 pathway. (A–D) Western blot analysis was performed to measure the protein levels of AVP, CaN, NFATC3, GPX4 and ACSL4. A colorimetric kit was used to measure the Fe2+ concentration (E) and MDA level (F). Data are expressed as the mean±SEM (n=3). ***P<0.001 vs the Ctrl group; #P<0.05, ##P<0.01, and ###P<0.001 vs the dDAVP+DMSO group.

Article Snippet: Colorimetric kits were employed to assess the total iron level (E-BC-K773-M; Elabscience, Wuhan, China), Fe 2+ concentration (E-BC-K881-M; Elabscience) and MDA concentration (E-BC-K025-S; Elabscience) according to the manufacturer’s instructions.

Techniques: In Vitro, Western Blot, Concentration Assay

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Arginine vasopressin induces ferroptosis to promote heart failure via activation of the V1aR/CaN/NFATC3 pathway

doi: 10.3724/abbs.2023289

Figure Lengend Snippet: Effects of NFATC3 on ferroptosis in HCM cells HCM cells were used for the in vitro experiments. IP (A) and luciferase activity (B) assays were performed to evaluate the binding between NFATC3 and ACSL4. sh-NFATC3 and sh-ACSL4 were used to inhibit NFATC3 and ACSL4 expression, respectively. (C–E) Western blot analysis was performed to measure the protein levels of NFATC3, GPX4 and ACSL4. A colorimetric kit was used to measure the Fe 2+ concentration (F) and MDA level (G). Data are expressed as the mean±SEM (n=3). ***P<0.001 vs the NC group; &&&P<0.001 vs the Ctrl group; ## P<0.01, ###P<0.001 vs the dDAVP+sh-NC group.

Article Snippet: Colorimetric kits were employed to assess the total iron level (E-BC-K773-M; Elabscience, Wuhan, China), Fe 2+ concentration (E-BC-K881-M; Elabscience) and MDA concentration (E-BC-K025-S; Elabscience) according to the manufacturer’s instructions.

Techniques: In Vitro, Luciferase, Activity Assay, Binding Assay, Expressing, Western Blot, Concentration Assay

Journal: iScience

Article Title: Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy

doi: 10.1016/j.isci.2024.109580

Figure Lengend Snippet:

Article Snippet: BC Assay: Protein Assay Kit , Interchim , Cat#UP40840A.

Techniques: Recombinant, Transfection, Electron Microscopy, Protease Inhibitor, Western Blot, Purification, SYBR Green Assay, Viability Assay, Sequencing, Software, Imaging